Stefan Hübner

Privatdozent


Institut für Anatomie
und Zellbiologie
 
Koellikerstr. 6
D-97070 Würzburg
 
Tel. +49 931 31 81311
Fax +49 931 31 82712
 
email
 

   


 

Forschungsbereiche:

   

· Molekulare Basis von Laminopathien
· Untersuchungen zur Funktion des karyophilen Adaptorproteins Kanadaptin
· Der Kernproteintransport in erkrankten Zellen
· Methoden zur Quantifizierung des nukleären Proteinimports/exports
 
 
 

Nukleärer Export eines grün fluoreszierenden Fusionsproteins mit Hilfe
der induzierten Heterodimerisierung (Busch et al., 2009)
 

Lehre:

   

· Kursus der makroskopischen Anatomie (WS)
· Seminar Anatomie mit klinischen Bezügen:
     Klinische Anatomie von Kopf und Sinnesorganen (WS)
· Topographische Anatomie (Teil) (WS)
· Integriertes Seminar:
     Anatomische Grundlagen neurologischer Erkrankungen (SS)
· Mikroskopisch anatomische Übungen (SS)
· Seminar Anatomie (SS)
· Anatomie I (Bewegungsapparat/Teil) (SS)
· Grundlagen klinisch-topographischer Anatomie:
     Spezialpräparationen von Hals, Brust und Bauch (SS)
· Krankheiten bei Zellkontakt- und Zytoskelettdefekten (SS)
· Repetitorium (Bauchsitus)
 

 
1983-1989 
 
  1988-1989 
 
 
1989-1992
1992 
 
 
 
1992-1993 
1993-1994 
 
1994-1997 
 
  1997-2003 
 
2003 
 
2003-2005 
2005-2006 
 
2006 
 
seit 1. Juli 2010

   

Beruflicher Werdegang:
Studium der Biologie an der Technischen Universität Darmstadt
Schwerpunkte: Biochemie und Mikrobiologie 
Diplomarbeit am Institut für Biochemie der Technischen Universität Darmstadt
Thema: “Konstruktion eines Expressionsvektors zur heterologen Genexpression
der humanen Antileukoprotease (ALP) in Aspergillus nidulans” 
Promotionsarbeit am Max-Planck Institut für Biophysik in Frankfurt 
Promotion an der Johann-Wolfgang von Goethe Universität Frankfurt
Thema: “Aufklärung der kompletten Primärstruktur des AE1-Proteins der
Forellenerythrozyten und Nachweis der funktionelle Expression in Oozyten
von Xenopus laevis”
MPG-Stipendiat am Max-Planck Institut für Biophysik in Frankfurt 
wissenschaftlicher Angestellter am Max Delbrück Zentrum für Molekulare Medizin
in Berlin Buch 
internationaler ARC und JCSMR Research Fellow an der John Curtin School of
Medical Research in Canberra, Australien
wissenschaftlicher Assistent am Institut für Anatomie und Zellbiologie der
Universität Würzburg 
Habilitation; Thema der Habilitationsschrift:
“Mechanismen des Kernproteintransportes” 
Research Fellow an der Monash University in Melbourne, Australien 
wissenschaftlicher Angestellter am Institut für Anatomie und Zellbiologie der
Universität Würzburg 
Akademischer Rat am Institut für Anatomie und Zellbiologie der Universität
Würzburg
Akademischer Oberrat am Institut für Anatomie und Zellbiologie der Universität
Würzburg

Publikationen:

Hübner S, Efthymiadis A (2012)
Recent progress in histochemistry and cell biology
Histochemistry and Cell Biology, DOI: 10.1007/s00418-012-0933-4

Hübner S, Efthymiadis A (2011)
Histochemistry and cell biology: the annual review 2010
Histochemistry and Cell Biology, DOI: 10.1007/s00418-011-0781-7

Filatova A, Leyerer M, Gorboulev V, Chintalapati C, Reinders Y, Müller TD, Srinivasan A,  Hübner S, Koepsell H (2009)
Novel Shuttling Domain in a Regulator (RSC1A1) of Transporter SGLT1 Steers Cell Cycle Dependent Nuclear Location.
Traffic, DOI: 10.1111/j.1600-0854.2009.00982.x

Busch A, Kiel T, Hübner S (2009)
Quantification of nuclear protein transport using induced heterodimerization.
Traffic, DOI: 10.1111/j.1600-0854.2009.00953.x

Busch A, Kiel T, Heupel WM, Wehnert M, Hübner S (2009)
Nuclear protein import is reduced in cells expressing nuclear envelopathy-causing lamin A mutants.
Experimental Cell Research 315 (2009), pp. 2373-2385

Hübner S, Eam JE, Wagstaff KM, Jans DA (2006)
Quantitative analysis of localization and nuclear aggregate formation induced by GFP-lamin A mutant proteins in living HeLa cells.
J. Cell. Biochem., 98, 810-826.
 
Hübner S, Eam JE, Hübner A, Jans DA (2006)
Laminopathy-inducing lamin A mutants can induce redistribution of lamin binding proteins into nuclear aggregates. Exp. Cell. Res., 312, 171-183.
 
Hübner S, Bahr C., Gößmann H, Efthymiadis A, and Drenckhahn D (2003)
Mitochondrial localization of the kidney adaptor protein kanadaptin in mammalian cells. Eur. J. of Cell Biol., 82, 240-52.
 
Hübner S, Jans DA, C-Y Xiao, AP John, and Drenckhahn D (2002)
Signal- and importin-dependent nuclear targeting of the kidney anion exchanger 1-binding protein kanadaptin.
Biochem. J., 361, 287-96.
 
Hübner S, Jans DA, Drenckhahn D (2001)
Roles of cytoskeletal and junctional plaque proteins in nuclear signaling. Int. Rev. Cytol. 208, 207-65.
 
Hübner S, Smith HMS, Hu W, Chan C-K, Rihs HP, Pascal B, Raikhel NV and Jans DA (1999)
Plant a-Importin binds nuclear localization sequences with high affinity and can mediate nuclear import in the absence of a ß-subunit. J. Biol. Chem., 274, 22610-22617.
 
Briggs LJ, Stein D, Goltz J, Corrigan VC, Efthymiadis A, Hübner S and Jans DA (1998)
The cAMP-dependent Protein Kinase Site (Ser312) Enhances Dorsal Nuclear Import through Facilitating Nuclear Localization Sequence/Importin Interaction. J. Biol. Chem., 273, 22745-22752.
 
Jans DA, Chan C-K, Hübner S (1998)
Signals mediating nuclear targeting and their regulation: Application in drug delivery. Med. Res. Rev. 18, 189-223.
 
Chan C-K, Hübner S, Hu W and Jans DA (1998)
Mutual exclusivity of DNA binding and nuclear localization signal recognition by the yeast transcription factor Gal4: Implications for non-viral DNA delivery. Gene Ther. 9, 1204-12.
 
Efthymiadis A, Shao H, Hübner S and Jans DA (1997)
Kinetic characterization of the human retinoblastoma protein bipartite nuclear localization sequence (NLS) in vivo and in vitro. A comparison with the SV40 large T-antigen NLS. J. Biol. Chem., 272, 22134-22139.
 
Xiao C-Y, Hübner S and Jans DA (1997)
SV40 large tumor-antigen nuclear import is regulated by the double-stranded DNA-dependent protein kinase site (Serine 120) flanking to the nuclear localization sequence. J. Biol. Chem., 272, 22191-22198.
 
Hübner S, Xiao C-Y and Jans DA (1997)
The casein kinase II site flanking the SV40 large T-antigen nuclear localisation sequence (NLS) enhances NLS binding by importin subunits.
J. Biol. Chem., 272, 17191-17195.
 
Xiao C-Y, Hübner S, Elliot R, Caon A and Jans DA (1996)
A consensus PK-A site in place of the CcN motif casein kinase II site of SV40 large T-antigen confers PK-A-mediated regulation of the rate of nuclear import. J. Biol. Chem., 271, 6451-6457.
 
Jans DA and Hübner S (1996)
Regulation of protein transport to the nucleus - the central role of phosphorylation. Physiol. Rev. 76, 651-685.
 
Hübner S, C-Y Xiao, J Reid, LJ Briggs, V Corrigan, P Jans, and DA Jans (1996)
"Protein transport to the nucleus and its regulation by phosphorylation" Today´s Life Science, January, 30-38.
 
Kollert-Joens A, Wagner S, Hübner S, Appelhans H and Drenckhahn D (1993)
Anion exchanger 1 in human kidney and oncocytoma differs from erythroid AE1 in its N-terminus by alternative splicing. Am. J. Physiol. 265, F813-821.
 
Karbach D, Hanke-Baier P, Wendel J, Svetina S, Müller W, Hübner S, Münchow T, Appelhans H and Passow H (1992)
Anion transport in the red blood cell of the mouse and in erythroleukemic cells. In: Progress in Cell Research, Vol. 2 (Passow, H. and Bamberg, E. Eds.), pp 295-305. Elsevier, Science Publishers B.V.
 
Hübner S, Michel F, Rudloff V and Appelhans H (1992)
Amino acid sequence of band 3 protein from rainbow trout red blood cells derived from the complementary cDNA. Biochem. J. 285, 17-23.
 
Hübner S, Weindel C, Michel F, Rudloff V and Appelhans H (1991)
Cloning the cDNA encoding the erythroid band 3 anion transporter of rainbow trout. In: Advances in Molecular Genetics 4, 219-227.
 

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